谁知道细菌培养基KMB的配方和用途啊,急用!谢谢
金氏培养基(Kings Medium)用于检测假单胞菌产生的青脓素。
KMB (Kings Medium B Agar)
20g Proteose Peptone No. 3 (Difco)
1.5g K2HPO4
0.738g MgSO4 (Anhydrous) or 1.5g MgSO4*7H2O
10ml Glycerol
990ml ddH2O
15g Bacto Agar
Mix Proteose Peptone, K2HPO4, MgSO4, and ddH2O till in solution. Add glycerol with a pipette. Rinse the pipette in the solution to remove residual glycerol from the inside of the pipette. Adjust pH to 7.4. Add agar and autoclave.
金氏培养基(甲)
蛋白胨20g,无水氯化镁1.4g,无水硫酸钾10g,琼脂15g,甘油10ml,蒸馏水1L。
将上述成分加热、溶解,调pH至7.2,分装试管,118~121℃灭菌15min,制成斜面和高层备用。